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KMID : 0613820170270030318
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Journal of Life Science
2017 Volume.27 No. 3 p.318 ~ p.324
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Anti-oxidant Function and Inhibitory Effects of the Expression of MITF, TRP-1, TRP-2 and Tyrosinase of Sesamum indicum L. in B16F10 Melanoma Cells
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Yoo Dan-Hee
Joo Da-Hye
Lee Jin-Young
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Abstract
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This study was performed to improve the antioxidant and skin-whitening activities of 70% ethanol extract from Sesamum indicum L. (SIL). The electron-donating ability of the SIL extract was 71.7% at a concentration of 1,000 μg/ml. The whitening effects that was measured by tyrosinase inhibition assay. As a result, SIL extract was shown 42% at 1,000 μg/ml concentration. The cell toxicity on B16F10 melanoma cells of SIL of 70% ethanol extract showed 84.3% at 1,000 μg/ml concentration. The microphthalmia-associated transcription factor (MITF), tyrosinase relate protein-1 (TRP-1), tyrosinase relate protein-2 (TRP-2) and Tyrosinase protein and mRNA expression inhibitory effect of SIL extract were measured by western blot and reverse transcription- polymerase chain reaction (PCR) at 50, 250, 500 μg/ml concentration. Consequently, the MITF, TRP-1, TRP-2, Tyrosinase protein expression inhibitory effect of SIL extract was decreased by 68.3%, 39.2%, 89.7%, 22.3%, respectively, at 500 μg/ml concentration. Moreover, MITF, TRP-1, TRP-2, Tyrosinase mRNA expression inhibitory effect by reverse-transcription-PCR of SIL extract was decreased by 81.8%, 66.5%, 84.2%, 68.1%, respectively, at 500 μg/ml concentration. Therefore, we excellently identified the antioxidant activities and whitening effect of SIL extract, and this finding suggested that SIL extract has great potential as a cosmetic ingredients.
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KEYWORD
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Antioxidant activities, Microphthalmia-associated transcription factor, Sesamum indicum L., tyrosinase related protein-1, tyrosinase related protein-2, Tyrosinase
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